American researchers have developed a promising new method for imaging DNA molecules, which is based on chemical labeling. It is surprisingly simple and effective, although it requires a deep understanding of the structure and functioning of DNA and RNA. Instead of observing complex molecules with a "gun eye", the team of Dr. Joshua Wanstein proposed to study their mutual arrangement and influence on each other.
As the doctor explains, the technique is not tied to any microscopic coordinate system, it scales without problems and allows you to immediately see the molecules in 3D. This is extremely important because you can quickly understand how cancer or immune cells interact with each other. And instead of a long search for targets, get a direct indication of the place where important events are taking place.
To implement the method, you need a bio-sample, a pipette and a reaction chamber. DNA markers are added to the grown cells, which themselves attach to specific RNAs, and serve as a kind of beacons about their location. Further, with the help of simple chemical reactions, markers are distributed throughout the sample. And, since we are talking about a living, active environment, as various processes proceed in it, we will see the movements of markers - clusters, single marks, tendencies to flow in a new direction, etc.
All complex processes in the interaction of cells are automatically visualized through DNA markers, which can be detected by chemical reactions. And it works great, even if the molecules are superimposed on each other, blocking the view from the outside. Scientists are left to observe the picture in order to sequence and decode the molecules for study at the right time.